Journal: BMC nephrology

Article Title: The glomerular parietal epithelial cell's responses are influenced by SM22 alpha levels.

doi: 10.1186/1471-2369-15-174

Figure Lengend Snippet: Figure 2 Transition cells in SM22 null mice are higher with experimental glomerular disease compared to wildtypes. A) Representative images of transition cells at × 630 original magnification. Glomerular epithelial transition cells defined PAX2 (red, nuclear) and SYNPO (green, cytoplasmic) double positive cells. SM22α +/+ is A1-A9. SM22α −/−is A10-A18. Day 0 (A1-A3, A10-A12), day 7 (A4-A6, A13-A15), day 14 (A7-A9, A16-A18) were reacted anti PAX2 antibody (Ab) (A1, A4, A7, A10, A13, A16) and anti Synaptopodin (SYNPO) Ab (A2, A5, A8, A11, A14, A17). The images were merged (A3, A6, A9, A12, A15, A18). The glomerular transition cells are on Bowman’s capsule (arrow) and in tuft (arrow head). B) Number of glomerular epithelial transition cells along BBM / Bowman’s capsule (BC) length (mm) is increased in SM22α −/−mice, compared with SM22α +/+ mice. C) Number of glomerular epithelial transition cells in tuft area / tuft area (μm2) is increased in SM22α −/+ −mice, compared with SM22α +/+ mice.

Article Snippet: The following primary antibodies were used: rabbit anti-rat paired box gene 2 (PAX2, a PEC nuclear protein) polyclonal antibody diluted 1:500 (Zymed Laboratories, South San Francisco, Calif., USA) and mouse anti- Synaptopodin (Synpo, a podocyte protein) monoclonal antibody, diluted 1:10 (Fitzgerald).

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